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atp1a1 plasmid donor rd agudelo et  (Addgene inc)


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    Structured Review

    Addgene inc atp1a1 plasmid donor rd agudelo et
    Atp1a1 Plasmid Donor Rd Agudelo Et, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atp1a1+plasmid+donor+rd/ATP1A1_plasmid_donor_RD+(Plasmid+%2386551)/pm41933972-267-97-102
    Average 93 stars, based on 6 article reviews
    atp1a1 plasmid donor rd agudelo et - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: Unconventional binding of Calmodulin to CHK2 kinase inhibits catalytic activity
    Article Snippet: .. 1 × 10 cells were co-transfected with 5 μg of eSpCas9-hGeminin –CHK2 gRNA plasmid, 5 μg of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551), and 2.5 μl ssODN donor (100 μM). .. To generate control cell lines, 1 × 10 cells were co-transfected with 5 μg of eSpCas9-hGeminin plasmid (Addgene plasmid, #86613) and 5 ug of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551).

    Article Title: Unconventional binding of Calmodulin to CHK2 kinase inhibits catalytic activity
    Article Snippet: 1 × 10 cells were co-transfected with 5 μg of eSpCas9-hGeminin –CHK2 gRNA plasmid, 5 μg of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551), and 2.5 μl ssODN donor (100 μM). .. To generate control cell lines, 1 × 10 cells were co-transfected with 5 μg of eSpCas9-hGeminin plasmid (Addgene plasmid, #86613) and 5 ug of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551). .. Cells were electroporated using the Neon Transfection System (Invitrogen, #MPK5000) at 1350 V, 20 ms, 2 pulse.

    Article Title: Unconventional binding of calmodulin to CHK2 kinase inhibits catalytic activity
    Article Snippet: .. 1 × 10 6 cells were co-transfected with 5 μg of eSpCas9-hGeminin –CHK2 gRNA plasmid, 5 μg of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551), and 2.5 μl ssODN donor (100 μM). .. To generate control cell lines, 1 × 10 6 cells were co-transfected with 5 μg of eSpCas9-hGeminin plasmid (Addgene plasmid, #86613) and 5 ug of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551).

    Article Title: Unconventional binding of calmodulin to CHK2 kinase inhibits catalytic activity
    Article Snippet: 1 × 10 6 cells were co-transfected with 5 μg of eSpCas9-hGeminin –CHK2 gRNA plasmid, 5 μg of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551), and 2.5 μl ssODN donor (100 μM). .. To generate control cell lines, 1 × 10 6 cells were co-transfected with 5 μg of eSpCas9-hGeminin plasmid (Addgene plasmid, #86613) and 5 ug of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551). .. Cells were electroporated using the Neon Transfection System (Invitrogen, #MPK5000) at 1350 V, 20 ms, 2 pulse.

    Control:

    Article Title: Unconventional binding of Calmodulin to CHK2 kinase inhibits catalytic activity
    Article Snippet: 1 × 10 cells were co-transfected with 5 μg of eSpCas9-hGeminin –CHK2 gRNA plasmid, 5 μg of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551), and 2.5 μl ssODN donor (100 μM). .. To generate control cell lines, 1 × 10 cells were co-transfected with 5 μg of eSpCas9-hGeminin plasmid (Addgene plasmid, #86613) and 5 ug of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551). .. Cells were electroporated using the Neon Transfection System (Invitrogen, #MPK5000) at 1350 V, 20 ms, 2 pulse.

    Article Title: Unconventional binding of calmodulin to CHK2 kinase inhibits catalytic activity
    Article Snippet: 1 × 10 6 cells were co-transfected with 5 μg of eSpCas9-hGeminin –CHK2 gRNA plasmid, 5 μg of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551), and 2.5 μl ssODN donor (100 μM). .. To generate control cell lines, 1 × 10 6 cells were co-transfected with 5 μg of eSpCas9-hGeminin plasmid (Addgene plasmid, #86613) and 5 ug of ATP1A1_plasmid_donor_RD (Addgene plasmid, #86551). .. Cells were electroporated using the Neon Transfection System (Invitrogen, #MPK5000) at 1350 V, 20 ms, 2 pulse.



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